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Molecular phylogeny of the hominoids: inferences from multiple independent DNA sequence data sets 总被引:14,自引:4,他引:10
Consensus on the evolutionary relationships of humans, chimpanzees, and
gorillas has not been reached, despite the existence of a number of DNA
sequence data sets relating to the phylogeny, partly because not all gene
trees from these data sets agree. However, given the well-known phenomenon
of gene tree-species tree mismatch, agreement among gene trees is not
expected. A majority of gene trees from available DNA sequence data support
one hypothesis, but is this evidence sufficient for statistical confidence
in the majority hypothesis? All available DNA sequence data sets showing
phylogenetic resolution among the hominoids are grouped according to
genetic linkage of their corresponding genes to form independent data sets.
Of the 14 independent data sets defined in this way, 11 support a human-
chimpanzee clade, 2 support a chimpanzee-gorilla clade, and one supports a
human-gorilla clade. The hypothesis of a trichotomous speciation event
leading to Homo; Pan, and Gorilla can be firmly rejected on the basis of
this data set distribution. The multiple-locus test (Wu 1991), which
evaluates hypotheses using gene tree-species tree mismatch probabilities in
a likelihood ratio test, favors the phylogeny with a Homo-Pan clade and
rejects the other alternatives with a P value of 0.002. When the
probabilities are modified to reflect effective population size differences
among different types of genetic loci, the observed data set distribution
is even more likely under the Homo-Pan clade hypothesis. Maximum-likelihood
estimates for the time between successive hominoid divergences are in the
range of 300,000-2,800,000 years, based on a reasonable range of estimates
for long-term hominoid effective population size and for generation time.
The implication of the multiple-locus test is that existing DNA sequence
data sets provide overwhelming and sufficient support for a
human-chimpanzee clade: no additional DNA data sets need to be generated
for the purpose of estimating hominoid phylogeny. Because DNA hybridization
evidence (Caccone and Powell 1989) also supports a Homo-Pan clade, the
problem of hominoid phylogeny can be confidently considered solved.
相似文献
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Transient increase in phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol trisphosphate during activation of human neutrophils 总被引:13,自引:0,他引:13
A E Traynor-Kaplan B L Thompson A L Harris P Taylor G M Omann L A Sklar 《The Journal of biological chemistry》1989,264(26):15668-15673
We recently showed that phosphatidylinositol trisphosphate (PIP3) was present in a unique lipid fraction generated in neutrophils during activation. Here, we demonstrate that the band containing this fraction isolated from thin layer chromatography consists primarily of PIP3 and that only small amounts of radiolabeled PIP3 exist prior to activation. In addition, high performance liquid chromatography of deacylated phospholipids from stimulated cells reveals an increase in a fraction eluting ahead of glycerophosphoinositol 4,5-P2. After removal of the glycerol we found that it coeluted with inositol 1,3,4-P3 when resubjected to high performance liquid chromatography. Thus, we have detected a second, novel form of phosphatidylinositol bisphosphate in activated neutrophils, PI-(3,4)P2. The elevation of PIP3 through the formyl peptide receptor is blocked by pretreatment with pertussis toxin, implicating mediation of the increase in PIP3 by a guanosine triphosphate-binding (G) protein. The rise in PIP3 is not secondary to calcium elevation. Buffering the rise in intracellular calcium did not diminish the increase in PIP3. The elevation of PIP3 appears to occur during activation with physiological agonists, its level varying with the degree of activation. Leukotriene B4, which elicits many of the same responses as stimulation of the formyl peptide receptor but with minimal oxidant production, stimulates a much attenuated rise in PIP3. Isoproterenol, which inhibits oxidant production also reduces the rise in PIP3. Hence formation of PI(3,4)P2 and PIP3 (presumed to be PI(3,4,5)P3) correlates closely with the early events of neutrophil activation. 相似文献
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Selective binding of L-thyroxine by myosin light chain kinase 总被引:3,自引:0,他引:3
L-Thyroxine selectively inhibited Ca2+-calmodulin-activated myosin light chain kinases (MLC kinase) purified from rabbit skeletal muscle, chicken gizzard smooth muscle, bovine thyroid gland, and human platelet with similar Ki values (Ki = 2.5 microM). A detailed analysis of L-thyroxine inhibition of smooth muscle myosin light chain kinase activation was undertaken in order to determine the effect of L-thyroxine on the stoichiometries of Ca2+, calmodulin, and the enzyme in the activation process. The kinetic data indicated that L-thyroxine does not interact with calmodulin but, instead, through direct association with the enzyme, inhibits the binding of the Ca2+-calmodulin complex to MLC kinase. L-[125I]Thyroxine gel overlay revealed that the 95-kDa fragment of chicken gizzard MLC kinase digested by chymotrypsin and all the fragments of 110, 94, 70, and 43 kDa produced by Staphylococcus aureus V8 protease digestion which contain the calmodulin binding domain retain L-[125I]thyroxine binding activity, whereas smaller peptides were not radioactive. Since MLC kinase is phosphorylated by cAMP-dependent protein kinase (2 mol of phosphate/mol of MLC kinase), the effect of L-thyroxine on the phosphorylation of MLC kinase also was examined. L-Thyroxine binding did not inhibit the phosphorylation of MLC kinase and, moreover, reversed the inhibition of phosphorylation obtained with the calmodulin-enzyme complex. These observations support the suggestion that L-thyroxine binds at or near the calmodulin-binding site of MLC kinase. L-Thyroxine may serve as a different type of pharmacological tool for elucidating the biological significance of MLC kinase-mediated reactions. 相似文献